CCK-8试剂盒

目录号 M4839

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CCK试剂盒简介

CCK试剂盒（Cell Counting Kit），为MTT 法的替代方法，是一种基于WST(水溶性四唑盐，化学名：2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)的广泛应用于细胞增殖和细胞毒性的快速高灵敏度检测试剂盒。

WST检测原理图

CCK法与其它检测方法之间的比较

CCK用途：药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验。

CCK试剂盒的优点及应用

CCK试剂盒使用方便，省去了洗涤细胞，不需要同位素和有机溶剂

CCK法的检测灵敏度很高，甚至可以测定较低细胞密度

CCK试剂盒为1瓶溶液，毋需预制，即开即用

CCK法的重复性优于MTT法

CCK试剂毒性

CCK和其他检测方法对比可以得出——CCK对细胞几乎无毒性，不伤害细胞，细胞可重复利用。

资料下载

CCK-8试剂盒使用说明书

	数据来源	J Hazard Mater (2020 Apr). Figure 6. Cell Counting Kit-8 (AbMole BioScience, Houston, TX, USA)
	方法	Cell viability assay
	细胞系	LO2 and HEK 293 cells
	浓度	-
	处理时间	-
	实验结果	As shown in Fig. 6, PAT-J significantly reduced the viability of the two cells compared with the JUICE group, while the cell viability of the PAT-JP and LS-J2 treated groups did not change significantly.
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	数据来源	Med Sci Monit (2018 Oct). Figure 2. Cell Counting Kit-8 (Abmole Bioscience, USA)
	方法	Cell viability assay
	细胞系	colon cancer cells
	浓度	10 nmol/L
	处理时间	1 h
	实验结果	Then, CCK8 assay was performed to measure the effect of MON1B on cell viability of colon cancer cells, which was inhibited time-dependently (12, 24, and 48 h) in the si-MON1B group, with significant differences at 24 and 48 h (P<0.05, Figure 2C).
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	数据来源	Sci Rep (2018;8:3891). Figure 4. CCK-8 (AbMole BioScience)
	方法	Cell viability assay
	细胞系	PLC5 cells
	浓度	10 nmol/L
	处理时间	48 h
	实验结果	In accordance with functional analysis, western blot confirmed that CCRK overexpression promoted cell proliferation by activating β-catenin/TCF signaling, which however significantly abrogated by bufalin
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	数据来源	Sci Rep (2018;8:3891). Figure 1. CCK-8 (AbMole BioScience)
	方法	Cell viability assay
	细胞系	PLC5 cells
	浓度	10 nmol/L
	处理时间	48 h
	实验结果	After 48 hours incubation, cell viability was measured by CCK-8 assay. In contrast with less influence on the growth of LO2 cells, bufalin exhibited strong ability to suppress the number of PLC5 cells in a dose-dependent manner.
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	数据来源	Cancer Biol Ther (2018 Jun 3;19(6):507-517). Figure 7. CCK-8 (Abmole Bioscience, Shanghai, China)
	方法	CCK-8 assay
	细胞系	C33A, Hcc94, HeLa, and SiHa cell lines
	浓度
	处理时间	72 h
	实验结果	As the results of the CCK-8 assay shown, the 72-hour treatment with buformin exhibited significant suppressive effects on cellular growth in C33A, Hcc94, and SiHa cells, but only exerted moderate effects in HeLa cells.
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	数据来源	Future Oncology (2018 Feb;14(3):241-253) . Figure 3. CCK-8 (AbMole BioScience, Shanghai, China)
	方法	Cell counting kit-8 assay
	细胞系	HeLa, SiHa, C33A and CasKi cells
	浓度	10 μl
	处理时间	2 h
	实验结果	Compared with its counterparts, knockdown of PXN suppressed cellular proliferation and induced cellular apoptosis
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	数据来源	PLoS One (2013). Figure 3. Cell counting kit-8
	方法	Cell proliferation assay
	细胞系	Human colon cancer cell lines HT-29, LOVO, and Caco-2
	浓度	10 μL/well
	处理时间	2 h
	实验结果	The effect of cPA on cell proliferation was determined using a colorimetric assay. We found that cPA inhibited the proliferation of HT-29 and LOVO cells but not of DLD-1 and Caco-2 cells, which have lower endogenous levels of PDE3B than HT-29 and LOVO cells.
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	数据来源	PLoS One (2013). Figure 4. Cell counting kit-8
	方法	Cell viability
	细胞系	Human mesenchymal stem cells
	浓度	20 µL/well
	处理时间	3 h
	实验结果	In group A, the cell number remained stable during the first 3 days, followed by a continuous increase till day 14. In group B, the cell number was stable during the first 2 days, then started to increase, and attained a plateau on day 12. In group C (control group), the cell number (Fig. 4A) remained stable during the first 4 days in culture, then started to increase, and plateaued on day 8.
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