CCK试剂盒简介
CCK试剂盒(Cell Counting Kit),为MTT 法的替代方法,是一种基于WST(水溶性四唑盐,化学名:2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)的广泛应用于细胞增殖和细胞毒性的快速高灵敏度检测试剂盒。
WST检测原理图
CCK法与其它检测方法之间的比较
CCK用途:药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验。
CCK试剂盒的优点及应用
CCK试剂盒使用方便,省去了洗涤细胞,不需要同位素和有机溶剂
CCK法的检测灵敏度很高,甚至可以测定较低细胞密度
CCK试剂盒为1瓶溶液,毋需预制,即开即用
CCK法的重复性优于MTT法
CCK试剂毒性
CCK和其他检测方法对比可以得出——CCK对细胞几乎无毒性,不伤害细胞,细胞可重复利用。
资料下载
2020 Aug.
Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study
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Life Sci. 2021 Jan 15;265:118853.
Circadian clock gene BMAL1 reduces urinary calcium oxalate stones formation by regulating NRF2/HO-1 pathway
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FEBS Open Bio. 2020 Dec 19.
Nicotinamide mononucleotide (NMN) protects bEnd.3 cells against H 2 O 2 -induced damage via NAMPT and the NF-κB p65 signalling pathway
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2020 Dec.
A Novel Circular RNA hsa_circRNA_103809/miR-377-3p/GOT1 Pathway Regulates Cisplatin-Resistance in Non-Small Cell Lung Cancer (NSCLC)
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2020 Aug.
Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study
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Onco Targets Ther. 2020 Sep 28;13:9543-9558.
Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder
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Life Sci. 2020 Oct 15;259:118180.
Upregulation of miR-133a-3p enhances Bufothionine-induced gastric cancer cell death by modulating IGF1R/PI3K/Akt signal pathway mediated ER stress
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J Appl Microbiol. 2020 Sep 29.
Enhanced in vitro antimicrobial activity of amphotericin B with berberine against dual-species biofilms of Candida albicans and Staphylococcus aureus
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Exp Physiol. 2020 Sep 22.
Involvement of the lncRNA AFAP1-AS1/microRNA-195/E2F3 axis in proliferation and migration of enteric neural crest stem cells of Hirschsprung's disease
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Cell Death Dis. 2020 Aug 20;11(8):664.
Long noncoding RNA TTN-AS1 facilitates tumorigenesis and metastasis by maintaining TTN expression in skin cutaneous melanoma
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Biochem Biophys Res Commun. 2020 Sep 10;530(1):47-53.
LncRNA Linc-PINT inhibits miR-523-3p to hamper retinoblastoma progression by upregulating Dickkopf-1 (DKK1)
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2020 Jun.
The Role of Transcription Factor Ap1 in The Activation of Nrf2/ARE Pathway Through TET1 in Diabetic Nephropathy
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Aging (Albany NY). 2020 Jun 9;12(11):11025-11041.
LncRNA ADAMTS9-AS2 inhibits gastric cancer (GC) development and sensitizes chemoresistant GC cells to cisplatin by regulating miR-223-3p/NLRP3 axis
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IUBMB Life. 2020 Jun 27.
Effects of genetic variations in Acads gene on the risk of chronic obstructive pulmonary disease
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World J Gastroenterol. 2020 Jun 7;26(21):2810-2820.
Gan Shen Fu Fang ameliorates liver fibrosis in vitro and in vivo by inhibiting the inflammatory response and extracellular signal-regulated kinase phosphorylation
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Open Life Sciences. 2020 Jun 30;15(1):409-417.
Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
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Anat Rec (Hoboken). 2020 Jan 21.
Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation
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Sci Adv. 2020 Jul 10;6(28):eaba7260.
A basal-bolus insulin regimen integrated microneedle patch for intraday postprandial glucose control
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Exp Biol Med (Maywood). 2020 Jun;245(11):925-932.
Circular RNA circ-CDYL sponges miR-1180 to elevate yes-associated protein in multiple myeloma
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Aging (Albany NY). 2020 May 4;12(9):8137-8150.
Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8
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Aging (Albany NY). 2020 Jun 9;12(11):11025-11041.
LncRNA ADAMTS9-AS2 inhibits gastric cancer (GC) development and sensitizes chemoresistant GC cells to cisplatin by regulating miR-223-3p/NLRP3 axis
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Cell Cycle. 2020 Aug;19(15):1857-1868.
Hsa_circ_0009910: oncogenic circular RNA targets microRNA-145 in ovarian cancer cells
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J Exp Clin Cancer Res. 2020 May 24;39(1):93.
TFEB-NF-κB Inflammatory Signaling Axis: A Novel Therapeutic Pathway of Dihydrotanshinone I in Doxorubicin-Induced Cardiotoxicity
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J Clin Lab Anal. 2020 Feb 28;e23249.
Retraction: Hsa_circ_0003645 Shows an Oncogenic Role by Sponging microRNA-1299 in Hepatocellular Carcinoma Cells
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Biomed Pharmacother. 2020 May 25;128:110244.
Emodin Succinyl Ester Inhibits Malignant Proliferation and Migration of Hepatocellular Carcinoma by Suppressing the Interaction of AR and EZH2
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J Cell Mol Med. 2020 Mar;24(5):2901-2916.
Verbascoside Inhibits Progression of Glioblastoma Cells by Promoting Let-7g-5p and Down-Regulating HMGA2 via Wnt/beta-catenin Signalling Blockade
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Vet Res. 2020 Apr 10;51(1):53.
Newcastle Disease Virus RNA-induced IL-1β Expression via the NLRP3/caspase-1 Inflammasome
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Saudi J Gastroenterol. 2020 May-Jun;26(3):144-152.
Inhibition of ALAS1 Activity Exerts Anti-Tumour Effects on Colorectal Cancer in vitro
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Naunyn Schmiedebergs Arch Pharmacol. 2020 Feb 8.
Knock-down of LncRNA-XIST Induced Glioma Cell Death and Inhibited Tumorigenesis by Regulating miR-137/SLC1A5 Axis-Mediated ROS Production
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Eur J Pharmacol. 2020 May 26;173212.
Pinocembrin Alleviates Glucocorticoid-Induced Apoptosis by Activating Autophagy via Suppressing the PI3K/Akt/mTOR Pathway in Osteocytes
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Colcom. 2020 Jul;Volume 37, 100268.
Antibacterial coatings based on microgels containing quaternary ammonium ions: Modification with polymeric sugars for improved cytocompatibility
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Chemosphere. 2020 Aug;253:126707.
Bisphenol A and Its Analogues Bisphenol S, Bisphenol F and Bisphenol AF Induce Oxidative Stress and Biomacromolecular Damage in Human Granulosa KGN Cells.
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Cell Cycle. 2020 May;19(10):1067-1076.
Hypoxia-induced Apoptosis of Cardiomyocytes Is Restricted by Ginkgolide B-downregulated microRNA-29.
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ACS Appl Mater Interfaces. 2020 Apr 29;12(17):19268-19276.
Universal Antibacterial Surfaces Fabricated From Quaternary Ammonium Salt-Based PNIPAM Microgels.
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Onco Targets Ther. 2020 May 4;13:3729-3737.
Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma.
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Biomed Pharmacother. 2020 May;125:109924.
MiR-130a Alleviated High-Glucose Induced Retinal Pigment Epithelium (RPE) Death by Modulating TNF-α/SOD1/ROS Cascade Mediated Pyroptosis.
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Carbohydr Polym. 2020 Jan 1;227:115314.
Structural characterisation and immunomodulatory activity of polysaccharides from white asparagus skin.
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Biofactors. 2020 Jan 8.
Propofol exerts neuroprotective functions by down-regulating microRNA-19a in glutamic acid-induced PC12 cells.
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Anat Rec (Hoboken). 2020 Jan 21.
Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation.
Cell Counting Kit-8购买自AbMole
Yonsei Med J. 2019 Sep;60(9):832-841.
miR-1301/TRIAP1 Axis Participates in Epirubicin-Mediated Anti-Proliferation and Pro-Apoptosis in Osteosarcoma.
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Oncol Lett. 2019 Nov;18(5):4613-4620.
MicroRNA-152 suppresses cisplatin resistance in A549 cells.
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Med Sci Monit. 2019 Aug 11;25:5977-5985.
Expression of circZNF609 is Down-Regulated in Colorectal Cancer Tissue and Promotes Apoptosis in Colorectal Cancer Cells by Upregulating p53.
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J Hazard Mater. 2020 Apr 15;388:121802.
Thiol-functionalized inactivated yeast embedded in agar aerogel for highly efficient adsorption of patulin in apple juice.
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J Clin Neurosci. 2019 Jun;64:234-241.
Potential contribution of microRNA-125b targeting p38MAPK to relieving intermittent hypoxia-induced dementia of rat models.
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Biochem Biophys Res Commun. 2019 Apr 30;512(2):218-223.
Versatile Pt NCs-based chemotherapeutic agents significantly induce the apoptosis of cisplatin-resistant non-small cell lung cancer.
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International Journal of Oncology. 2019 Mar;929-941.
lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3.
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Int J Pharm. 2018 Dec 13;556:338-348.
Self-implanted tiny needles as alternative to traditional parenteral administrations for controlled transdermal drug delivery.
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Med Sci Monit. 2018 Oct 28;24:7710-7718.
Knockdown of MON1B Exerts Anti-Tumor Effects in Colon Cancer In Vitro.
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Sci Rep. 2018 Mar 1;8(1):3891.
Bufalin suppresses hepatocarcinogenesis by targeting β-catenin/TCF signaling via cell cycle-related kinase
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Cancer Biol Ther. 2018 Feb 5.
Buformin suppresses proliferation and invasion via AMPK/S6 pathway in cervical cancer and synergizes with paclitaxel
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Future Oncology 2018 Jan 10.
The overexpression of PXN promotes tumor progression and leads to radioresistance in cervical cancer
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