IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293.
别名：Interleukin-23; IL-23A; IL-23 alpha & IL-12 beta
IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293. The IL-23 alpha protein has cytokine activity and binds to interleukin-23 receptors. It regulates cytokine production, lymphocyte activation, and peptidyl-tyrosine phosphorylation. It influences T cell proliferation and RNA polymerase II transcription. Found in the extracellular space, it is part of the interleukin-23 complex. It is mainly expressed in the thymus, spleen, and other tissues. It is orthologous to the human IL23A gene encoding interleukin 23 subunit alpha.
The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. A DNA sequence encoding the rat IL23A (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat IL12B (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified.
Measured by its ability to induce IL17 secretion by mouse splenocytes, the ED50 for this effect is 0.5-3ng/mL.
Endotoxin < 1 EU/µg
Apparent Molecular Weight: approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE
Lyophilized from sterile PBS, pH 7.4.
| 性状 | Lyophilized powder |
| 溶解性（25°C） | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
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