蛋白G是一种细菌细胞壁蛋白,在某些C组和G组链球菌菌株的细胞表面表达。蛋白G是一种检测IgG的强大试剂。蛋白G被用于Western印迹分析,以检测硝酸纤维素膜上的各种抗原-抗体复合物。此外,蛋白G被广泛用作亲和层析法中与树脂耦合的配体,用于抗体纯化。
蛋白G是一种细菌细胞壁蛋白,在某些C组和G组链球菌菌株的细胞表面表达。蛋白G是一种检测IgG的强大试剂。蛋白G被用于Western印迹分析,以检测硝酸纤维素膜上的各种抗原-抗体复合物。此外,蛋白G被广泛用作亲和层析法中与树脂耦合的配体,用于抗体纯化。蛋白G对人IgG的Fab-和Fc-片段都有亲和力,有独立的结合点。通过非免疫机制与几个物种的免疫球蛋白的Fc区结合,对几乎所有哺乳动物的免疫球蛋白G(IgG)类表现出极大的亲和力,包括所有人类IgG亚类(IgG1、IgG2、IgG3和IgG4)以及兔、鼠和山羊IgG。蛋白G与所有测试的单克隆IgG结合,包括小鼠IgG1、IgG2a和IgG3,以及大鼠IgG2a、IgG2b和IgG2c。此外,来自人、牛、兔、山羊、大鼠和小鼠的多克隆IgG与蛋白G结合,而鸡的IgG却没有。蛋白G也被证明能与人血清白蛋白结合。
分子量:~31kD
剂型描述:液体或冻干粉
储存条件 | Storage at -20℃ or below. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
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