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Recombinant Human NT-3 (E.coli) 重组人NT-3蛋白 (E.coli)

目录号 M15079 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


利用大肠杆菌表达系统制备了重组人神经营养因子-3,并表达了编码Tyr139-Thr257的目的基因。Accession #: P20783。

Recombinant Human NT-3 (E.coli)结构式

别名:Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2

规格 价格 库存状态
10ug ¥ 2970 中国库存现货
50ug ¥ 8820 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
生物活性

种属:Human

表达系统:E.coli

纯度:还原SDS-PAGE测定大于95%

内毒素:小于0.001 ng/ug (0.01 EU/ug)

存储及稳定性:冻干蛋白应在< -20°C保存,但在室温下可保存3周。重组蛋白溶液可在4-7℃下保存2-7天。重组样品的等分液在< -20°C下稳定3个月。


生物活性:神经营养因子-3 (NT-3)是神经营养因子NGF家族的成员之一,在结构上与β-NGF、BDNF和NT-4相关。NT3 cDNA编码一个含有信号肽的257个氨基酸残基前体蛋白和一个原蛋白,经裂解得到119个氨基酸残基的NT3成熟蛋白。成熟的人、小鼠和大鼠NT-3的氨基酸序列是相同的。NT-3选择性地促进中枢神经系统和周围神经系统中特定神经元亚群的分化和生存。

化学性质
溶解性(25°C) It is not recommended to reconstitute to a concentration less than 100μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] D R Safina, et al. Bioorg Khim. [Oligomeric organization of recombinant human neurotrophins expressed in Escherichia coli cells]

[2] Haidi Zhang, et al. Cell. Single processing center models for human Dicer and bacterial RNase III

[3] J O Hui, et al. J Protein Chem. Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein

[4] A Shintani, et al. Biochem Biophys Res Commun. Monoclonal antibodies against human neurotrophin-3

[5] S Honda, et al. Arch Biochem Biophys. Escherichia coli-derived human interferon-gamma with Cys-Tyr-Cys at the N-terminus is partially N alpha-acylated

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