Recombinant Human Mucin-1/MUC1 Protein (HEK293, C-6His) 别名：CD227; EMA; Tumor-associated mucin; Cancer antigen 15-3; CA 15-3; 重组人Mucin-1/MUC1 蛋白 (HEK293, C-6His)
Mucin 1, known as MUC1, is a multifunctional protein expressed on the apical surface of most epithelial cells. MUC1/Muc1 in promotes cell growth and survival. MUC1 does have an exceptionally strong glycan-dependent apical targeting signal that can redirect a basolaterally expressed protein to the apical cell surface in polarized epithelia. Mucin-1/MUC1 protein and its α subunit have dual functions of adhesion and anti-adhesion proteins, forming a protective layer on epithelial cells.
化学性质/溶解性/储存
| 基因ID | 4582 [NCBI] |
| 性状 | Lyophilized powder |
| 中文名称 | 重组人Mucin-1/MUC1 蛋白 (HEK293, C-6His) |
| 溶解性（仅列举部分溶剂） | Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
*不同实验中用到的溶剂可能不同，具体实验所需溶剂及溶解方法请参考相关文献描述。
生物活性
Mucin 1, known as MUC1, is a multifunctional protein expressed on the apical surface of most epithelial cells. MUC1/Muc1 in promotes cell growth and survival. MUC1 does have an exceptionally strong glycan-dependent apical targeting signal that can redirect a basolaterally expressed protein to the apical cell surface in polarized epithelia. Mucin-1/MUC1 protein and its α subunit have dual functions of adhesion and anti-adhesion proteins, forming a protective layer on epithelial cells. At the same time, the β subunit and its C-terminal domain participate in cell signaling through phosphorylation and protein-protein interactions.
Recombinant Human Mucin-1 expressed the target gene encoding Ser890-Gly1158 with a 6His tag at C-terminus.
Recombinant Human Mucin-1 is present at 1 μg/mL can bind Anti-Mucin-1 antibody, the ED50 for this effect is 5.9 ng/mL.
Accession: P15941-1
Apparent Molecular Weight: (35-75)&(9-11) KDa, reducing conditions
Endotoxin < 1 EU/µg
Tag Info: C-6His
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
