Recombinant Human HK2 Protein (Baculovirus-Insect, C-His) 别名：Hexokinase 2; HKII Protein; 重组人HK2 蛋白 (Baculovirus-Insect, C-His)
Hexokinase 2 (HK2) is a rate-determining enzyme in aerobic glycolysis, a process upregulated in tumor cells. HK2 expression is controlled by various transcription factors and epigenetic alterations and is heterogeneous in hepatocellular carcinomas (HCCs).
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人HK2 蛋白 (Baculovirus-Insect, C-His) |
| 溶解性 | Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Hexokinase 2 (HK2) is a rate-determining enzyme in aerobic glycolysis, a process upregulated in tumor cells. HK2 expression is controlled by various transcription factors and epigenetic alterations and is heterogeneous in hepatocellular carcinomas (HCCs). HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic processes.
The recombinant Human HK2 consists of 928 amino acids and predicts a molecular mass of 103.84 kDa. A DNA sequence encoding the Human HK2 (P52789) (Met1-Arg917) was expressed with a polyhistidine tag at the C-terminus.
Accession: P52789
Apparent Molecular Weight: 103.37 kDa, under reducing conditions
Endotoxin < 1 EU/µg
Lyophilized from sterile 20mM PB, 300mM Nacl, 10% glycerol, pH 7.0.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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