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Recombinant Human SCF (P. pastoris) 重组人SCF蛋白 (P. pastoris)

目录号 M11493 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


重组人SCF蛋白剂量依赖地刺激TF-1细胞,ED50 < 2.0 ng/ml,对应的比活性为5 × 10 5IU/mg。蛋白构造:与N端的甲硫氨酸共表达。Accession #:P21583-1。

Recombinant Human SCF (P. pastoris)结构式

别名:Kit ligand; C-Kit Ligand; Mast Cell Growth Factor

规格 价格 库存状态
10ug ¥ 1900 中国库存现货
50ug ¥ 3100 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 1 EU/μg
  • COA
  • MSDS
生物活性

表达系统:P. pastoris

纯度:SDS-PAGE分析> 95%

内毒素水平:凝胶凝血法测定蛋白质< 1 EU/μg

表观分子量:~ 20kda,在SDS-PAGE还原条件下

存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。


生物活性:干细胞因子 (P. pastoris) (也被称为SCF 、kit配体、KL或钢因子)是一种与c-KIT受体(CD117)结合的细胞因子。SCF既可以作为跨膜蛋白存在,也可以作为可溶性蛋白存在。在骨髓培养中,它能刺激髓系、红细胞和淋巴系祖细胞的增殖,并已被证明与菌落刺激因子协同作用。SCF在胚胎发育的造血过程中起着重要的作用。SCF可以调节骨髓干细胞龛内的造血干细胞。SCF已被证明能提高体外造血干细胞的存活率,并有助于造血干细胞在体内的自我更新和维持。

化学性质
溶解性(25°C) Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Laura L Thompson, et al. Int J Mol Sci. The SCF Complex Is Essential to Maintain Genome and Chromosome Stability

[2] Gitana Mickiene, et al. PeerJ. Human granulocyte-colony stimulating factor (G-CSF)/stem cell factor (SCF) fusion proteins: design, characterization and activity

[3] Zeyuan Cao, et al. Stem Cell Res Ther. Hepatocyte growth factor (HGF) and stem cell factor (SCF) maintained the stemness of human bone marrow mesenchymal stem cells (hBMSCs) during long-term expansion by preserving mitochondrial function via the PI3K/AKT, ERK1/2, and STAT3 signaling pathways

[4] Danrui Cui, et al. Cell Rep. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation

[5] Moein Dehbashi, et al. Mol Biol Res Commun. Comparative genomics of human stem cell factor (SCF)

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