重组人SCF蛋白剂量依赖地刺激TF-1细胞,ED50 < 2.0 ng/ml,对应的比活性为5 × 10 5IU/mg。蛋白构造:与N端的甲硫氨酸共表达。Accession #:P21583-1。
别名:Kit ligand; C-Kit Ligand; Mast Cell Growth Factor
表达系统:P. pastoris
纯度:SDS-PAGE分析> 95%
内毒素水平:凝胶凝血法测定蛋白质< 1 EU/μg
表观分子量:~ 20kda,在SDS-PAGE还原条件下
存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。
生物活性:干细胞因子 (P. pastoris) (也被称为SCF 、kit配体、KL或钢因子)是一种与c-KIT受体(CD117)结合的细胞因子。SCF既可以作为跨膜蛋白存在,也可以作为可溶性蛋白存在。在骨髓培养中,它能刺激髓系、红细胞和淋巴系祖细胞的增殖,并已被证明与菌落刺激因子协同作用。SCF在胚胎发育的造血过程中起着重要的作用。SCF可以调节骨髓干细胞龛内的造血干细胞。SCF已被证明能提高体外造血干细胞的存活率,并有助于造血干细胞在体内的自我更新和维持。
溶解性(25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
储存条件 |
粉末型式 -20°C 3年;4°C 2年 溶于溶剂 -80°C 6个月;-20°C 1个月 |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
[4] Danrui Cui, et al. Cell Rep. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation
[5] Moein Dehbashi, et al. Mol Biol Res Commun. Comparative genomics of human stem cell factor (SCF)
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