Insulin (human) 是一种多肽激素,其调节血液中的糖(葡萄糖)水平并且由胰岛的β细胞产生。
Insulin (human) 是一种多肽激素,其调节血液中的糖(葡萄糖)水平并且由胰岛的β细胞产生。
*该产品在溶液状态不稳定,建议您现配现用, 尽快使用
This product solution cannot be autoclaved.
It is also soluble in 125mM NaHCO3. Alkaline storage solutions are not recommended as high pH increases deamidation and protein aggregation.
General recommendation:
1. Do not prepare concentrated insulin solution because of poor solubility and the formation of polymers.
2. Adjust acidic pH to promote solutization and minimize the effects of neutral ph-binding proteases. For added protection, acid protease inhibitors may be considered.
3. The solution was aseptically filtered using a 0.2 micron low protein binding filter. Avoid aeration and/or blistering during solubilization and filtration as this may lead to protein denaturation.
4. Store at 2-8°C for 8 hours.
5. Do not heat more than 30°C and do not freeze.
6. Recommended working concentration of insulin is 1-10 μg/mL.
As an alternative, insulin solutions can be formulated with phosphate buffers (PBS). The above products were dissolved in 1mg /mL PBS solution. Shake gently at room temperature for 1-2 hours. No whirlpool. The solution is ready for cleanup. Prior to evaluation, follow steps 3-6 for final processing.
Cell Commun Signal. 2022 Jul 7;20(1):102.
分子量 | 5807.57 |
分子式 | C257H383N65O77S6 |
CAS号 | 11061-68-0 |
溶解性(25°C) | Water 10 mg/mL (Need ultrasonic and warming and adjust pH to 2-3 with HCl) |
储存条件 | -20°C, protect from light, dry, sealed |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前,需考虑其在不同溶剂中的溶解度限制。
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 0.1722 mL | 0.8609 mL | 1.7219 mL |
5 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL |
10 mM | 0.0172 mL | 0.0861 mL | 0.1722 mL |
小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 | |
重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km 系数 | 3 | 6 | 12 | 8 | 5 | 20 |
动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
动物 A的Km系数 |
例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。
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