HEPES 4-羟乙基哌嗪乙磺酸

生物活性

HEPES是可满足于所有生物学研究的最佳缓冲液之一。HEPES 是一种非挥发的两性离子化学缓冲剂，在 pH 值为 6.8 至 8.2 时为有效的缓冲液，广泛应用于细胞培养。

使用AbMole产品发表的文献

• Nucleic Acids Res. 2022 Aug 12;50(14):7938-7958.

  HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia

• Acta Pharm Sin B. 2022 Aug.

  Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1highCD11b+ cells elimination in hepatocellular carcinoma

• Cell Commun Signal. 2022 Jul 7;20(1):102.

  Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells

• Nat Commun. 2021 Jun 23;12(1):3896.

  Single cell derived mRNA signals across human kidney tumors

• EMBO Mol Med. 2021 Jul 7;13(7):e13067.

  Cysteamine-bicalutamide combination therapy corrects proximal tubule phenotype in cystinosis

• Adv Healthc Mater. 2021 Aug;10(16):e2100821.

  Extracellular Matrix Stiffness Regulates DNA Methylation by PKCα-Dependent Nuclear Transport of DNMT3L

• Cell Death Dis. 2021 Feb 15;12(2):179.

  Targeting transcription of MCL-1 sensitizes HER2-amplified breast cancers to HER2 inhibitors

• Cells. 2021 Feb 18;10(2):430.

  Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis

• Cell Stem Cell. 2020 Nov 5;27(5):840-851.e6.

  Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo

• Nat Commun. 2020 Aug 27;11(1):4279.

  Caveolin-1-mediated sphingolipid oncometabolism underlies a metabolic vulnerability of prostate cancer

• Environ Health Perspect. 2020 Jun;128(6):67008.

  Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation

• Sci Rep. 2020 Jul 31;10(1):12991.

  Mutation accumulation and developmental lineages in normal and Down syndrome human fetal haematopoiesis

• J Virol. 2020 Feb 14;94(5):e01791-19.

  Targeting Kaposi's Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use

• NAS. 2020 Jun.

  Multiscale activity imaging in mammary gland reveals how oxytocin enables lactation

化学性质

分子量	238.3
分子式	C8H18N2O4S
CAS号	7365-45-9
溶解性（25°C）	Water ≥ 200 mg/mL
储存条件	粉末型式       -20°C   3年；4°C   2年 溶于溶剂       -80°C   6个月；-20°C   1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.1964 mL	20.982 mL	41.9639 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Pierce, et al. J Med Chem. Docking study yields four novel inhibitors of the protooncogene Pim-1 kinase.