Cycloheximide 放线菌酮

生物活性

Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

使用AbMole产品发表的文献

• Adv Sci (Weinh). 2024 Jan;e2207435.

  Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage

• Mol Carcinog. 2024 Jan.

  SN-38, an active metabolite of irinotecan, inhibits transcription of nuclear factor erythroid 2-related factor 2 and enhances drug sensitivity of colorectal cancer cells

• iScience. 2024 Jan 19.

  FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression

• Mol Cell Biochem. 2024 Feb 17.

  Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling

• J Transl Med. 2023 May 5;21(1):303.

  SYTL2 promotes metastasis of prostate cancer cells by enhancing FSCN1-mediated pseudopodia formation and invasion

• Cell Death Discov. 2023 Apr 26.

  Ammonium Tetrathiomolybdate Relieves Oxidative Stress in Cisplatin-induced Acute Kidney Injury via NRF2 Signaling Pathway

• Cell Death Discov. 2023 Jul 25;9(1):259.

  Ammonium tetrathiomolybdate relieves oxidative stress in cisplatin-induced acute kidney injury via NRF2 signaling pathway

• J Cell Mol Med. 2023 Aug 29.

  Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling

• FASEB J. 2023 Oct;37(10):e23183.

  IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6A-dependent manner

• New Journal of Chemistry. 2023 Jun 20.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection

• New J. Chem. 2023 Jun 28;47, 14344-14354.

  Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection

• Biochem Genet. 2023 Nov 11.

  Functional Characterization of a Novel SLC4A4 Variant and Uniparental Isodisomy in Proximal Renal Tubular Acidosis Patient

• Cell Death Differ. 2022 Jul;29(7):1395-1408.

  SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK

• Research Square. 2022 Oct 12.

  IGF2BP2 promotes ovarian cancer growth and metastasis via upregulating CKAP2L protein expression in a m6A-dependent manner

• Ecotoxicol Environ Saf. 2022 Jun 1;237:113564.

  Environmental BPDE induced human trophoblast cell apoptosis by up-regulating lnc-HZ01/p53 positive feedback loop

• Virol Sin. 2022 Aug 16;S1995-820X(22)00143-2.

  CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells

• Cell Biol Toxicol. 2022 Apr;38(2):291-310.

  Lnc-HZ08 regulates BPDE-induced trophoblast cell dysfunctions by promoting PI3K ubiquitin degradation and is associated with miscarriage

• Bioengineered. 2022 Apr;13(4):8000-8012.

  UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization

• J Biol Chem. 2022 Nov 21;102734.

  Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer

• Mol Cell Endocrinol. 2022 Apr 5;545:111575.

  An intron mutation of HNF1A causes abnormal splicing and impairs its activity as a transcription factor

• Adv Sci (Weinh). 2021 Feb.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple‐Negative Breast Cancer Tumor Growth

• Adv Sci (Weinh). 2021 Jan 6;8(4):2003205.

  Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple-Negative Breast Cancer Tumor Growth

• Clin Transl Med. 2021 Apr;11(4):e395.

  FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression

• Int J Mol Sci. 2021 Aug 16;22(16):8779.

  Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency

• J Cancer. 2021 Oct 17;12(23):7041-7051.

  N6-methyladenosine regulates ATM expression and downstream signaling

• Cell Rep. 2020 Jul 28;32(4):107974.

  L ARP7 Is a BRCA1 Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis

• Cell Rep. 2020 Jul 28;32(4):107974.

  LARP7 is a BRCA1 ubiquitinase substrate and regulates genome stability and tumorigenesis

• Theranostics. 2020 Apr 27;10(13):5671-5686.

  SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma

• Cell Oncol (Dordr). 2020 Oct 19.

  Ubiquitin-specific protease 2a promotes hepatocellular carcinoma progression via deubiquitination and stabilization of RAB1A

• Cell Microbiol. 2020 Mar;22(3):e13148.

  Hepatitis B virus X protein modulates upregulation of DHX9 to promote viral DNA replication.

• Life Sci. 2020 Sep 15;257:118021.

  TRIB3 destabilizes tumor suppressor PPARα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia

• Front Cell Infect Microbiol. 2020 Apr 16;10:158.

  Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels

• Aging (Albany NY). 2018 Oct 18;10(10):2783-2799.

  Ubiquitin-specific protease 4 promotes metastasis of hepatocellular carcinoma by increasing TGF-β signaling-induced epithelial-mesenchymal transition.

• Biotechnol Lett. 2018 Aug;40:1181-1188.

  UHRF2 promotes DNA damage response by decreasing p21 via RING finger domain.

• Protein Cell. 2017 Mar;8(3):202-218.

  E3 ligase UHRF2 stabilizes the acetyltransferase TIP60 and regulates H3K9ac and H3K14ac via RING finger domain

产品使用成果展示

	数据来源	Biotechnol Lett (2018 Aug). Figure 3. cycloheximide (Abmole Bioscience)
	方法	Western blotting
	细胞系/动物模型	Human embryonic kidney 293 cells
	浓度	50 ug/mL
	处理时间	3 h
	实验结果	The overexpression of UHRF2 shortened the half-life of p21 to 1 h compared to 3 h under controlled conditions.

	数据来源	Protein and Cell (2016). Figure 4.CHX (ABmole Bioscience, USA)
	方法	Half-life experiments
	细胞系/动物模型	Human normal liver cells (LO2)
	浓度	100 μg/mL
	处理时间
	实验结果	Graph represents collated results rom three independent half-life experiments performed in HEK293, LO2 and HepG2 cells. TIP60 levels were normalized to 1 at time zero.

化学性质

分子量	281.35
分子式	C15H23NO4
CAS号	66-81-9
溶解性（25°C）	DMSO ≥ 80 mg/mL Water 15 mg/mL (ultrasonic and warming)
储存条件	4°C, protect from light
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.5543 mL	17.7715 mL	35.5429 mL
5 mM	0.7109 mL	3.5543 mL	7.1086 mL
10 mM	0.3554 mL	1.7771 mL	3.5543 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Teklebirhan G,et.al. Int J Microbiol. Prevalence of Dermatophytic Infection and the Spectrum of Dermatophytes in Patients Attending a Tertiary Hospital in Addis Ababa, Ethiopia.