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Cidofovir 西多福韦

目录号 M1695 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


Cidofovir是一种具有抗病毒活性的化合物,通过选择性抑制病毒DNA的合成而抑制病毒复制。Cidofovir还具有抑制肿瘤生长的活性,可用于肿瘤的相关研究。

Cidofovir结构式

别名:Vistide; GS 0504; HPMPC

规格 价格 库存状态
Free Sample (0.5-1 mg)  ¥ 0 中国库存现货
5mg ¥ 234 中国库存现货
10mg ¥ 423 中国库存现货
50mg ¥ 810 中国库存现货
100mg ¥ 1314 中国库存现货
其他规格数量报价?

质量标准及产品资料
生物活性

Cidofovir通过选择性抑制病毒DNA的合成而抑制病毒复制。Cidofovir处理培养的细胞,抑制人类巨细胞病毒(HCMV)感染。Cidofovir抑制巨细胞病毒(CMV)蚀斑形成,即使是感染后48小时后加入到细胞中,作用于Davis和AD-169株时IC50分别为0.9 μg/mL和1.6 μg/mL。 Cidofovir也抑制单纯疱疹病毒感染。

使用AbMole产品发表的文献
产品使用成果展示
数据来源 Towson University thesis(2015) . Figure 12. cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
方法 Plaque Assay
细胞系/动物模型 Equine dermal fibroblast (E. Derm) cells
浓度 7.5 µg/mL
处理时间 4 d
实验结果 Well plates pre-treated with 7.5 µg/mL CDV contained some uninfected cells.
数据来源 Towson University thesis(2015) . Figure 7 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
方法 MTT assay
细胞系/动物模型 Equine dermal fibroblast (E. Derm) cells
浓度 1, 2.5, 5, 7.5, and 10 µg/mL
处理时间 24 h
实验结果 The combination of CDV and ACV at these chosen concentrations was found to be non-toxic for the cells since the lowest observed cell viability was around 78%.
数据来源 Towson University thesis(2015) . Figure 6 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
方法 MTT assay
细胞系/动物模型 Equine dermal fibroblast (E. Derm) cells
浓度 1, 5, 12.5, and 17.5 µg/mL
处理时间 24 h
实验结果 "CDV combined with FOS at these chosen concentrations were found to be non-toxic for the cells since the lowest observed cell viability was around 85%."
数据来源 Towson University thesis(2015) . Figure 4 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
方法 MTT assay
细胞系/动物模型 Equine dermal fibroblast (E. Derm) cells
浓度 1, 5, 10, 15, 20, 25, and 30 µg/mL
处理时间 24 h
实验结果 CDV’s CC50 value was around 27.5 µg/mL.
实验参考
体外实验*
细胞系 Madin–Darby Canine Kidney or Vero cells
方法 In vitro efficacy of cidofovir against CHV-1 Madin–Darby Canine Kidney or Vero cells were plated into 96-well plates at 1*104 cells/well and maintained in Dulbecco’s minimal essential medium with 1 g/L glucose, l-glutamine, and sodium pyruvate (Cell Grow; Corning, Manassas, VA) containing 10% fetal bovine serum (Atlanta Biological, Flowery Branch, GA) and penicillin (200 U/mL)/ streptomycin (200 mg/mL) (Life Technologies, Grand Island, NY) for 24 h until confluent. Monolayers were infected with 5 PFU/well CHV-1 (a previously described field strain that was also utilized during the in vivo experimental study evaluating cidofovir) or HSV-1 strain F, respectively. After 2 h of incubation at 37 C, virus supernatants were removed, cells were rinsed once with phosphate-buffered saline (PBS), and 100 mL of sequential 2-fold serial dilutions of cidofovir (starting at 625 mM) was added to each well. Infection controls without cidofovir and noninfected drug-treated controls were also included. Plates were incubated at 37 C until a cytopathic effect (CPE) was visible in the infected nontreated controls, which was *72 hours postinfection (hpi) for CHV-1 and 48 hpi for HSV-1, respectively. Plates were rinsed 3 times with PBS, fixed in 80% ethanol for 10 min at -20 C, and stained with crystal violet (Fisher Scientific, Fair Lawn, NJ). Virusinduced CPE was scored as the percent of wells per dilution with observable plaque formation, and EC50 values were calculated using GraphPad Prism.
浓度 0~625µM
处理时间 72 hours postinfection (hpi) for CHV-1 and 48 hpi for HSV-1

*上述方法来自公开文献,仅供相同目的实验参考。如实验目的、材料、方法不同,请参考其他文献。

体内实验*
动物模型 Recurrent ocular CHV-1 infection in dogs by administration of systemic prednisolone (3.0 mg/kg PO q24 h) for 7 consecutive days beginning on study day 1.
配制 0.9% sodium chloride solution
剂量 1 drop of 0.5% cidofovir ophthalmic solution
给药处理 eyes drop

*上述方法来自公开文献,仅供相同目的实验参考。如实验目的、材料、方法不同,请参考其他文献。

化学性质
分子量 279.19
分子式 C8H14N3O6P
CAS号 113852-37-2
溶解性(25°C) DMSO
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前,需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.5818 mL 17.909 mL 35.8179 mL
5 mM 0.7164 mL 3.5818 mL 7.1636 mL
10 mM 0.3582 mL 1.7909 mL 3.5818 mL
不同实验动物依据体表面积的等效剂量转换表(参考来源于公开文献
小鼠 大鼠 豚鼠 仓鼠
重量 (kg) 0.02 0.15 1.8 0.4 0.08 10
体表面积 (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km 系数 3 6 12 8 5 20
动物 A (mg/kg) = 动物 B (mg/kg) ×  动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Mateos et al. J Pediatr Hematol Oncol. Successful Response to Cidofovir of Adenovirus Hepatitis During Chemotherapy in a Child With Hepatoblastoma.

[2] Mikolajczak et al. Eur Arch Otorhinolaryngol. The impact of cidofovir treatment on viral loads in adult recurrent respiratory papillomatosis.

[3] Israely et al. Virol J. A single cidofovir treatment rescues animals at progressive stages of lethal orthopoxvirus disease.

[4] Muluneh et al. J Oncol Pharm Pract. Successful clearance of cutaneous acyclovir-resistant, foscarnet-refractory herpes virus lesions with topical cidofovir in an allogeneic hematopoietic stem cell transplant patient.

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关键词:Cidofovir, Vistide; GS 0504; HPMPC, Cidofovir供应商, Anti-infection抑制剂, 购买Cidofovir, Cidofovir溶解度, Cidofovir结构式








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