Carboplatin 卡铂

生物活性

Carboplatin是一种DNA合成抑制剂，结合到DNA上，干扰细胞修复机制。Carboplatin作用于一组人类卵巢癌细胞系，包括A2780, SKOV3,和 IGROV-1细胞，抑制细胞增殖,IC50分别为6.1 μM, 12.4 μM 和2.2 μM。

实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）

细胞实验
细胞系	5637 cells
方法	Cells were dosed and incubated with 290 μM carboplatin or 290 μM carboplatin combined with 0.08 μM paclitaxel for 4 h, each including 0.43 μM[14C]carboplatin (106 dpm/ml). After washing, cells were maintained with 14C carboplatin-free culture media. DNA was extracted from cells and converted to graphite and measured by AMS for 14C quantification as previously described.
浓度	290 μM
处理时间	4 h

动物实验
动物模型	Female 6‐week‐old nude mice
配制
剂量	100 mg/kg
给药处理	s.c.

不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将白藜芦醇用于小鼠的剂量22.4 mg/kg 换算成大鼠的剂量，需要将22.4 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到白藜芦醇用于大鼠的等效剂量为11.2 mg/kg。

化学数据

储备液配制

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.6936 mL	13.468 mL	26.936 mL
5 mM	0.5387 mL	2.6936 mL	5.3872 mL
10 mM	0.2694 mL	1.3468 mL	2.6936 mL

使用AbMole产品发表的文献

• 

  Int J Oncol. 2020 Jan;56(1):47-68.

  Circulating non‑coding RNA‑biomarker potential in neoadjuvant chemotherapy of triple negative breast cancer?
  Carboplatin购买自AbMole

• 

  Cell Cycle. 2018;17(10):1235-1244.

  Apatinib, a novel tyrosine kinase inhibitor, suppresses tumor growth in cervical cancer and synergizes with Paclitaxel.
  Carboplatin购买自AbMole

• 

  Cell Death Dis. 2018 Feb 12;9(2):213.

  Autophagy mediates glucose starvationinduced glioblastoma cell quiescence and chemoresistance through coordinating cell metabolism, cell cycle, and survival
  Carboplatin购买自AbMole

客户产品验证及相关生物数据

	数据来源	Cell Cycle (2018 Jul). Figure 5. Carboplatin (Abmole Bioscience)
	方法	cellular proliferation assay
	细胞系	SiHa cells
	浓度	5 μM
	处理时间	-
	实验结果	However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94)

	数据来源	Cell Death Dis (2018). Figure 3. Carboplatin (Abmole Bioscience)
	方法	Glucose starvation sensitizes glioblastoma cells to chemotherapies
	细胞系	U87 and U251 cells
	浓度	50 μM
	处理时间	5 d
	实验结果	Treatment with either temozolomide or carboplatin induced about 50 and 80% GBM cell death under normal and glucose starvation conditions, respectively

	数据来源	Cell Death Dis (2018). Figure 1. Carboplatin (Abmole Bioscience)
	方法	Glucose starvation sensitizes glioblastoma cells to chemotherapies
	细胞系	U87 and U251 cells
	浓度	50 μM
	处理时间	5 d
	实验结果	The cytotoxic effect was progressive and by day 5, temozolomide or carboplatin treatment caused 40–60% cell loss in U87 and U251 cells. Glucose starvation nearly doubled the cell loss to 70–90%.

	数据来源	Mol Oncol (2014). Figure 4. Carboplatin
	方法	s.c.
	细胞系	Female 6-week-old nude mice
	浓度	100 mg/kg
	处理时间	5 weeks
	实验结果	Both carboplatin treatment groups showed significantly reduced tumor burden as compared to the untreated controls from weeks 4 to 10 (P < 0.0001)

参考文献

Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity.
Jiang S, et al. Chem Res Toxicol. 2015 Dec 21;28(12):2250-2. PMID: 26544157.

Enhancement of paclitaxel and carboplatin therapies by CCL2 blockade in ovarian cancers.
Moisan F, et al. Mol Oncol. 2014 Oct;8(7):1231-9. PMID: 24816187.

Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes
Matthew D Hall, et al. Cancer Res. 2014 Jul 15;74(14):3913-22. PMID: 24812268.