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Anti-human CD28 mAb 抗人CD28单克隆抗体

目录号 M10603 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


CD28分子存在于大多数T细胞表面,被认为是一种T细胞特有的表面分子。T细胞对抗原的反应性主要通过CD3/TCR复合物介导,但也有赖于T细胞表面其他分子的协同。

Anti-human CD28 mAb结构式
规格 价格 库存状态
500ug ¥ 2205 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >98%, Endotoxin<0.1EU/μg
  • COA
  • MSDS
生物活性

CD28分子存在于大多数T细胞表面,被认为是一种T细胞特有的表面分子。T 细胞可通过 CD3/TCR、CD2 和 CD28 等多种途径被有效激活。从外周血、骨髓或脐血中分离出的单个核细胞在 CD3、CD28 单克隆抗体和多种细胞因子存在的条件下,经过一定时间培养可以获得具有肿瘤细胞杀伤活性的 CIK(Cytokine-inducedKiller)细胞。


表达宿主(Expression host)

杂交瘤细胞


浓度(Concentration) 1mg/mL


使用说明(Instructions)

可溶法: 推荐使用浓度为500ng/ml,在该浓度条件下细胞可获得充分的活化和刺激,细胞扩增倍数和最终的CD3+CD56+双阳率有明显提高。

包被法: 10mL PBS缓冲液(5ug/mL anti-human CD28)铺T75方瓶置于4℃过夜。使用前去除PBS缓冲液,生理盐水洗涤三次。

存 储:2-8℃可保存3个月,长期存储置于-20℃或-80℃保存,建议开封后分装成小份,避免反复冻融。

化学性质
溶解性(25°C) PBS
储存条件 2-8°C for 3 months, long-term storage at -20°C or -80°C
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Shin Foong Ngiow, et al. Cancer Res. Agonistic CD40 mAb-Driven IL12 Reverses Resistance to Anti-PD1 in a T-cell-Rich Tumor

[2] Stephen L Shiao, et al. Transplantation. Immunomodulatory properties of FK734, a humanized anti-CD28 monoclonal antibody with agonistic and antagonistic activities

[3] Stamatis-Nick C Liossis, et al. BioDrugs. Costimulation blockade in the treatment of rheumatic diseases

[4] Philip Tan, et al. J Immunol. "Superhumanized" antibodies: reduction of immunogenic potential by complementarity-determining region grafting with human germline sequences: application to an anti-CD28

[5] R S Lee, et al. J Immunol. Blockade of CD28-B7, but not CD40-CD154, prevents costimulation of allogeneic porcine and xenogeneic human anti-porcine T cell responses

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