CD28分子存在于大多数T细胞表面,被认为是一种T细胞特有的表面分子。T细胞对抗原的反应性主要通过CD3/TCR复合物介导,但也有赖于T细胞表面其他分子的协同。
CD28分子存在于大多数T细胞表面,被认为是一种T细胞特有的表面分子。T 细胞可通过 CD3/TCR、CD2 和 CD28 等多种途径被有效激活。从外周血、骨髓或脐血中分离出的单个核细胞在 CD3、CD28 单克隆抗体和多种细胞因子存在的条件下,经过一定时间培养可以获得具有肿瘤细胞杀伤活性的 CIK(Cytokine-inducedKiller)细胞。
表达宿主(Expression host)
杂交瘤细胞
浓度(Concentration) 1mg/mL
使用说明(Instructions)
可溶法: 推荐使用浓度为500ng/ml,在该浓度条件下细胞可获得充分的活化和刺激,细胞扩增倍数和最终的CD3+CD56+双阳率有明显提高。
包被法: 10mL PBS缓冲液(5ug/mL anti-human CD28)铺T75方瓶置于4℃过夜。使用前去除PBS缓冲液,生理盐水洗涤三次。
存 储:2-8℃可保存3个月,长期存储置于-20℃或-80℃保存,建议开封后分装成小份,避免反复冻融。
溶解性(25°C) | PBS |
储存条件 | 2-8°C for 3 months, long-term storage at -20°C or -80°C |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
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