Acetylcysteine (NAC) N-乙酰-半胱氨酸；乙酰半胱氨酸

生物活性

Acetylcysteine(NAC; N-acetyl-l-cysteine)是ROS抑制剂，拮抗多种蛋白酶体抑制剂的活性。它还是肿瘤坏死因子TNF的抑制剂，主要用作祛痰剂，可处理扑热息痛(对乙酰氨基酚)过量。Acetylcysteine(N-acetyl-l-cysteine) 通过抑制IκB kinases抑制TNF诱导的NF-κB活化。Acetylcysteine(N-acetyl-l-cysteine) 通过线粒体依赖性途径诱导凋亡并抑制铁死亡和病毒复制。

使用AbMole产品发表的文献

• Cell Metab. 2024 Jan;S1550-4131(23)00477-1.

  Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment

• Biomed Pharmacother. 2024 Feb 23;173:116304..

  Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

• Inflamm. Res. 2024 Feb;73, 175-181.

  The immunomodulatory efects of metformin in LPS‑induced macrophages: an in vitro study

• Asian Journal of Pharmaceutical Sciences. 2023 Nov.

  Mechanistic engineering of celastrol liposomes induces ferroptosis and apoptosis by directly targeting VDAC2 in hepatocellular carcinoma

• Sci Total Environ. 2023 Dec;30:913:169730.

  Selenium deficiency exacerbated Bisphenol A-induced intestinal toxicity in chickens: Apoptosis and cell cycle arrest mediated by ROS/P53

• Front Immunol. 2023 Mar 14;14:1120996.

  ROS-AMPK/mTOR-dependent enterocyte autophagy is involved in the regulation of Giardia infection-related tight junction protein and nitric oxide levels

• Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127501.

  Bisphenol A exposure exacerbates tracheal inflammatory injury in selenium-deficient chickens by regulating the miR-155/TRAF3/ROS pathway

• Free Radic Biol Med. 2023 Mar 27;202:46-61.

  Mitochondrial GRIM-19 loss in parietal cells promotes spasmolytic polypeptide-expressing metaplasia through NLR family pyrin domain-containing 3 (NLRP3)-mediated IL-33 activation via a reactive oxygen species (ROS) -NRF2- Heme oxygenase-1(HO-1)–NF–кB axis

• J Cell Physiol. 2023 Apr 3.

  Bisphenol A aggravate selenium deficiency‐induced apoptosis via miR‐215‐3p/Dio1 to activate ROS/PI3K/AKT pathway in chicken arterial

• J Cell Physiol. 2023 Jun 3.

  miR-210/NF-κB axis: A new direction for regulating cadmium-induced pig artery inflammatory injury

• J Photochem Photobiol B. 2023 Jun 5;245:112735.

  The AMPK-dependent inhibition of autophagy plays a crucial role in protecting photoreceptor from photooxidative injury

• Chin J Nat Med. 2023 Sep 6;21(10): 759-774.

  Paeonol reduces microbial metabolite α-hydroxyisobutyric acid to alleviate the ROS/TXNIP/NLRP3 pathway-mediated endothelial inflammation in atherosclerosis mice

• Acta Pharm Sin B. 2022 May;12(5):2300-2314.

  Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1

• J Hazard Mater. 2022 Oct 5;439:129502.

  Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation

• J Pharm Anal. 2022 Aug;12(4):570-582.

  18beta-Glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs

• Neurochem Res. 2022 Oct 30.

  MiR-29c Inhibits TNF-α-Induced ROS Production and Apoptosis in Mouse Hippocampal HT22 Cell Line

• Environ Toxicol. 2022 Oct;37(10):2552-2565.

  Polystyrene nanoplastics exacerbated lipopolysaccharide‐induced necroptosis and inflammation via the ROS/MAPK pathway in mice spleen

• Redox Biol. 2021 Aug;44:102003.

  Roles of selenoprotein S in reactive oxygen species-dependent neutrophil extracellular trap formation induced by selenium-deficient arteritis

• Acta Pharm Sin B. 2021 Nov 10.

  Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1

• Ecotoxicol Environ Saf. 2021 Oct 1;222:112488.

  H2S exposure induces cell death in the broiler thymus via the ROS-initiated JNK/MST1/FOXO1 pathway

• Front Oncol. 2021 May 26;11:679816.

  Antidiabetic Agent DPP-4i Facilitates Murine Breast Cancer Metastasis by Oncogenic ROS-NRF2-HO-1 Axis via a Positive NRF2-HO-1 Feedback Loop

• Front Oncol. 2021 Sep 24;11:728047.

  Antidiabetic DPP-4 Inhibitors Reprogram Tumor Microenvironment That Facilitates Murine Breast Cancer Metastasis Through Interaction With Cancer Cells via a ROS–NF-кB–NLRP3 Axis

• J Infect Dis. 2021 Oct 28;224(8):1333-1344.

  Distinct Mitochondria-Mediated T-Cell Apoptosis Responses in Children and Adults With Coronavirus Disease 2019

• Pathogens. 2020 Aug 23;9(9):693.

  Giardia duodenalis Induces Apoptosis in Intestinal Epithelial Cells via Reactive Oxygen Species-Mediated Mitochondrial Pathway In Vitro

产品使用成果展示

	数据来源	Pathogens 2020 Aug. Figure 5. N-acetyl-l-cysteine (Abmole Bioscience Inc, Houston, TX, USA)
	方法	Cell Viability Assay
	细胞系/动物模型	Caco-2 cells
	浓度	10 mM/well
	处理时间	6 h
	实验结果	It was shown in Figure 5A that pre-incubation of Caco-2 cells with NAC for 3 h before exposure to trophozoites could block the release of Cyt-c. The activation of CASP3 was diminished by NAC in trophozoites-treated cells

化学性质

分子量	163.19
分子式	C5H9NO3S
CAS号	616-91-1
溶解性（25°C）	DMSO 30 mg/mL Water 30 mg/mL
储存条件	粉末型式       -20°C   3年；4°C   2年 溶于溶剂       -80°C   6个月；-20°C   1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.1278 mL	30.6391 mL	61.2783 mL
5 mM	1.2256 mL	6.1278 mL	12.2557 mL
10 mM	0.6128 mL	3.0639 mL	6.1278 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Qiuting Zhang, et al. J Cancer. Artemisinin Derivatives Inhibit Non-small Cell Lung Cancer Cells Through Induction of ROS-dependent Apoptosis/Ferroptosis

[2] Stutzman D, et al. Am J Health Syst Pharm. Acetylcysteine for treatment of autism spectrum disorder symptoms.